cd45 pe cy7 bd  (Miltenyi Biotec)

Journal: Stem cells (Dayton, Ohio)

Article Title: Combination therapy with Treg and MSC enhances potency and attenuation of inflammation after traumatic brain injury compared to monotherapy

doi: 10.1002/stem.3320

Figure Lengend Snippet: (A): Representative samples from both sham and CCI groups are shown. The single cell population was identified based on SSC and FSC. Live cells were identified as negative for the 7-AAD. Microglia were identified using a two-step method. First, CD11b/c+ cells (PE-Cy7) were selected to identify all myeloid cells. The CD11b/c+ cells were then gated on P2Y12 (FITC) and CD45 (APC-Cy7). Microglia were identified as triple positive cells. SSC, side scatter; FSC, forward scatter. (B): The multiple color flow cytometry panel used to identify and immunophenotype microglia and peripheral myeloid cells.

Article Snippet: SSC, side scatter; FSC, forward scatter. (B): The multiple color flow cytometry panel used to identify and immunophenotype microglia and peripheral myeloid cells. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Fluorochrome Antibody Clone Vendor APC-Cy7 CD45 OX-1 BD PE-Cy7 CD11b/c OX-42 BD FITC P2Y12 n/a Alomone Labs PE CD32 D34–485 BD Alexa Fluor 647 RT1B OX-6 BD PerCP Cy5.5 7-AAD n/a BD Open in a separate window Multicolor Flow Cytometry Microglia/Myeloid Cell Panel Microglia gating strategy Conventional flow cytometry analyses were performed with FlowJo vr10.6.1.

Techniques: Flow Cytometry

Journal: Stem cells (Dayton, Ohio)

Article Title: Combination therapy with Treg and MSC enhances potency and attenuation of inflammation after traumatic brain injury compared to monotherapy

doi: 10.1002/stem.3320

Figure Lengend Snippet: Multicolor Flow Cytometry Microglia/Myeloid Cell Panel

Article Snippet: SSC, side scatter; FSC, forward scatter. (B): The multiple color flow cytometry panel used to identify and immunophenotype microglia and peripheral myeloid cells. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Fluorochrome Antibody Clone Vendor APC-Cy7 CD45 OX-1 BD PE-Cy7 CD11b/c OX-42 BD FITC P2Y12 n/a Alomone Labs PE CD32 D34–485 BD Alexa Fluor 647 RT1B OX-6 BD PerCP Cy5.5 7-AAD n/a BD Open in a separate window Multicolor Flow Cytometry Microglia/Myeloid Cell Panel Microglia gating strategy Conventional flow cytometry analyses were performed with FlowJo vr10.6.1.

Techniques: Flow Cytometry

Journal: Scientific Reports

Article Title: Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients

doi: 10.1038/s41598-021-86875-y

Figure Lengend Snippet: Gating strategies of flow cytometric analysis of PTPRG protein and its expression during the treatment plan. ( a ) Gating strategies of PTPRG expression on the sub-population of white blood cells. Neutrophils (red color) have an intermediate level of CD45 and high side-scattered light (SSC); Monocytes (blue color) have a slightly higher level of CD45 expression and intermediate SSC while lymphocytes (green color) have the highest level of expression of CD45 but the lowest level of SSC. Doublets discrimination and exclusion of dead cells using 7-AAD staining (red rectangle) allow an easier identification of positively stained populations. In comparison the lower population was the target of part of the leukemic stem cell. ( b ) Neutrophils and Monocytes showed a low level of PTPRG expression at the time of diagnosis. PTPRG restored its expression, at least in part of the sub-population of white blood cells followed by TKIs therapy. Of note, lymphocytes remained at a low level acting as an internal control. Follow up time points F1, F2 and F3 were 3, 6, and 12 months of successful TKIs as per ELN timelines. The MFI values were obtained by calculating the ratio differences between the signals derived from the signal of mAB TPγ B9-2 and irrelevant mouse IgG1. ( c ) Gating strategy to identify leukemic CD34+CD38− stem cells. For myeloid progenitors and its sub-population, we targeted 15–20% upper and lower population of CD34 (red color) with CD38, with the upper population corresponding to the target of interest (hematopoietic stem cells). In comparison the lower population correspond to part of the leukemic stem cell.

Article Snippet: The FACs tubes were placed on ice, and 5 μl CD45 primary antibody (Becton Dickinson international company (BD)-PE-CY7 anti-Human mouse Catalog No. 557748), 5 μl of CD34 antibody (BD-BV421 mouse anti-Human Catalog No. 562577), and 20 μl CD38 antibody (BD-APC labeled anti-Human Catalog No. 555462) were added and incubated in the dark for 20 min at 4 °C according to supplier’s recommendations.

Techniques: Expressing, Staining, Comparison, Biomarker Discovery, Control, Derivative Assay